Comparison of allele frequencies between hexaploid triticale cultivars and a world collection of bread (Triticum aestivum) and durum (Triticum durum) wheat was investigated at Glu-A1 and Glu-B1: only a significant association was found for Glu-A1 alleles (χ2 = 2.26, p =0.36) between triticale and bread wheat. On the basis of allelic frequencies at the seven loci, genetic distances between hexaploid triticales grouped according to their origins revealed two groups: winter triticales mostly originating from European germplasm and spring triticales essentially of CIMMYT origin. Glu-B1 and Glu-B3 loci were the most polymorphic with 9 alleles each. Analysis by SDS-PAGE of the majority of hexaploid triticales (69 cultivars) grown in France allowed to identify 36 alleles at seven loci: Glu-A1, Glu-B1, Glu-R1, Gli-R2, Glu-B2, Glu-A3 and Glu-B3. Although the endosperm storage protein of hexaploid triticale have already been analysed, the allelic diversity of glutenins and secalins remains to be described.
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